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- W2370810189 abstract "A novel RT-PCR approach based on the specific upstream/downstream primers of YLSR 462 YLSF111 was developed for detecting asymptomatic yellow leaf syndrome (YLS) of sugarcane breeding lines and commercial varieties. Of the 140 parental and commercial clones from Louisiana and Texas of USA and other parts of the world, only six were YLSLV (YLS Luteovirus) free as detected by RT-PCR and DAS-ELISA. Higher Brix readings were associated with symptom expression (8.4) and a high level of YLS (100%), and lower average Brix reading (4.2) often involved asymptomatic expression and a lower level of YLS detection by both RT-PCR and DAS-ELISA. Some (55.17%) of asymptomatic crossing progenies/cultivars with lower Brix readings as assayed appeared to be YLS positive, indicating that Brix readings alone are not reliable enough for YLS detection. RT-PCR proved more sensitive than DAS-ELISA for detecting pre-symptom sugarcane leaf of YLS. Ninety-five percent of the crossing lines and varieties of USA used in this experiment were susceptible to YLSLV." @default.
- W2370810189 created "2016-06-24" @default.
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- W2370810189 date "2001-01-01" @default.
- W2370810189 modified "2023-09-25" @default.
- W2370810189 title "DIAGNOSIS AND IDENTIFICATION OF PRE-SYMPTOMS OF SUGARCANE YLS WITH RT-PCR AND DAS-ELISA" @default.
- W2370810189 hasPublicationYear "2001" @default.
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