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- W2370854757 abstract "The objectives of this study were to optimize the dissociation and culture conditions of protoplast of Medicago varia and establish the protoplast fusion for forming somatic hybridization system.The tested material was hypocotyl callus of Russian variegated alfalfa.And the influences of enzyme combinations,osmotic regulation content,enzymolysis time and callus derived time for hydrolysis protoplast and the influences of the hormone concentration and the protoplasts density for protoplasts culture were studied.The results showed that the optimum enzyme combinations were 2% cellulose onnzuka R-10+0.5%Pectinase Y-23+0.3% Macerozyme R-10,the optimum mannitol concentration was 0.55mol/L,the optimum enzymolysis time was 12 hours and the optimum callus subculture time was 14 days;in the solid-liquid comprehensive cultivation mode,the optimum hormone combination was 1.5mg/L 2,4-D+1.0mg/L 6-BA and the optimum culture density was 3×105/mL." @default.
- W2370854757 created "2016-06-24" @default.
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- W2370854757 date "2013-01-01" @default.
- W2370854757 modified "2023-09-26" @default.
- W2370854757 title "Optimization of Dissociation and Culture Conditions of Protoplasts of Callus of Russian Variegated Alfalfa" @default.
- W2370854757 hasPublicationYear "2013" @default.
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