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- W2371012380 abstract "The SPF chicken whole blood,peripheral blood mononuclear cells(PBMCs)and splenic lymphocytes from low-speed centrifugation of heparinized blood,PBMCs and splenic lymphocytes by Ficoll gradient centrifugation procedure,were prepared and stimulated by mitogens and CpG ODN 10 and 11,respectively.The stimulated lymphocytes were cultured at different temperatures and different cell concentrations.The transformation effect of the stimulated lymphocytes was further measured by using 3H-TdR incorporation assay to determine and compare the stimulation index(SI)of the stimulated lymphocytes from different procedures and conditions.And the chicken lymphocyte transformation assay based on plastic flat with 96 wells and suitable for large scale screening of CpG ODNs with strong immunostimulatory activity was established.And the library of 38 CpG ODNs designed and synthesized were screened by using the above established assay.The results showed as follows: The chicken PBMCs at the concentration of 10 7 mL -1 by low-speed centrifugation procedure can respond effectively and stably to the stimulation of CpG ODN 10 and 11 for 64 66 h at 37 ℃ 5% CO 2 incubation ;The ODNs without CpG motif have no stimulation activity on chicken lymphocytes; CpG ODNs 10,11,14 have the stronger stimulation activity,SI10." @default.
- W2371012380 created "2016-06-24" @default.
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- W2371012380 date "2004-01-01" @default.
- W2371012380 modified "2023-09-23" @default.
- W2371012380 title "Screening CpG oligodeoxynucleotides(ODNs)with strong immunostimulatory activity on chicken in vitro by lymphocyte transformation assay" @default.
- W2371012380 hasPublicationYear "2004" @default.
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