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- W2371033797 abstract "Objective To observe the effect of recombinant adeno-associated virus(rAAV)-mediated klotho(KL) gene expression on kidney fibrosis in rats with type 2 diabetes mellitus(T2DM)as well as the relevant mechanism. Methods Male SD rats were randomly divided into four groups,of which three groups(T2DM-mKL,T2DM-GFP and T2DM-PBS)were selected for establishment of T2DM model. The rats in T2DM-mKL,T2DM-GFP and T2DM-PBS groups were injected i. v. with rAAV. mKL,rAAV. GFP and PBS respectively,while those in normal contro(lSD-PBS)group with PBS. The rats were killed 12 weeks later,of which the kidney tissue specimens were collected,prepared into frozen section and observed for expression of GFP by fluorescent microscopy,for pathological change and expression of collagen fiber by Masson staining,for expressions of KL and vimentin(VIM)by immunohistochemistry,for transcription level of mRNA of Rho associated coiled-coilforming protein kinase(ROCK)gene by RT-PCR,and for ROCKⅠ protein activity by Western blot.Results GFP was highly expressed in kidney tissues of rats in T2DM-GFP and T2DM-mKL groups. The structure of glomerular basement membrane of rats in T2DM-mKL group was clear and intact,while the numbers of collagen fibers in mesangial and tubulointerstitial domains decreased significantly as compared with those in T2DM-PBS and T2DM-GFP groups. The expression level of KL in T2DM-mKL group was significantly higher than those in other groups(P 0. 01),while that of VIM was lower than those of other groups and showed significant difference with those in T2DM-PBS and T2DM-GFP groups(P 0. 01). Both the ROCKI mRNA transcription level and ROCK Ⅰ activity in T2DM-mKL group were significantly lower than those in T2DM-PBS group(both P 0. 05). Conclusion The rAAV. mKL transfection significantly increased the KL expression in kidneys of rats with T2DM,delayed the progression of renal fibrosis in diabetes, of which hthe mechanism might be associated with the inhibition of ROCK signaling pathway by KL." @default.
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- W2371033797 date "2013-01-01" @default.
- W2371033797 modified "2023-09-28" @default.
- W2371033797 title "Effect of recombinant adeno-associated virus-mediated klotho gene expression on kidney fibrosis in rats with type 2 diabetes mellitus and relevant mechanism" @default.
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