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- W2371042563 abstract "Objective To explore the culture conditions and the biological behaviors of human laryngeal epithelial cells. Methods The explants coming from healthy fetuses of induced labor of the middle and late periods were cultured with 2% serum. The biological features of the cultured cells were observed by the reverse microscope, transmission electron microscope and cytokeratins imrnunohistochemistry,Results The success rate of explant outgrowth was 47 % ± 11 %. The outgrowth could be seen after 3 days and gradually covered the whole bottom of the flask after 10 -14 days. The cells displayed a flat polygonal shape, contained tonofilaments and expressed cytokeratin. Desmosomal junctions were seen between cells. Conclusion The culture conditions used in the experiment are suitable for the primary culture of human laryngeal epithelial cells. The cultured cells will facilitate further research on etiology and pathogenesis of larynx carcinoma because of their proliferative and differentiative potency." @default.
- W2371042563 created "2016-06-24" @default.
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- W2371042563 date "2001-01-01" @default.
- W2371042563 modified "2023-09-24" @default.
- W2371042563 title "Primary culture of human laryngeal epithelial cells" @default.
- W2371042563 hasPublicationYear "2001" @default.
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