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- W2371055512 abstract "[Objective]The study aimed to investigate the carotenoid level change and cbp gene expression character in silkworm larvae. [Method]The silk glands of 3th,4th,5th instar larvae of silkworm with yellow and green cocoon strains were selected to extract their total DNA by referring to the reagent introduction of Takara Trizol plus and the special primer was designed on base of cbp gene sequence provided by the literature.The mRNA expression character of cbp gene and the change of carotenoid level in the main tissue of silkworm larvae with different colored cocoon strains at different instars was investigated by RT-PCR method.[Result]The mRNA expression of cbp gene could keep some level in 3th and 5th instar larvae of different silkworm strains and was almost undetectable in 4th instar larvae.The UV-Vis spectrum study on the carotenoid level in the same tissue of silkworm showed that the carotenoid content in the silk glands of 4th instar silkworm was very low. The mRNA expressions of cbp gene were different among different colored cocoon strains.Only one mRNA transcript lacking exon 2 was found in green cocoon strains and a complete mRNA transcript was expressed in all yellow cocoon strains.[Conclusion]The mRNA expression of cbp gene was correlated to the carotenoid level in silkworm." @default.
- W2371055512 created "2016-06-24" @default.
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- W2371055512 date "2009-01-01" @default.
- W2371055512 modified "2023-09-24" @default.
- W2371055512 title "Correlation of cbp Gene Expression in Silkworm with Carotenoid Level in Larvae" @default.
- W2371055512 hasPublicationYear "2009" @default.
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