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- W2371057206 abstract "Objective To compare stability, accuracy and suitability of Light Gycler and high prescure liquid chromatography in detecting mole percent guanine plus cytosine content (GC%).Methods Light Cycler method, was used following the melting curves and the values of Tm were obtained from the computer screen of Light Cycler, to calculate the GC% according to the formula: GC%x=GC%r - 8.567 (Tmx Tmr) + 0.066 (Tmx 2 Tmr 2). The GC% of bacterial chromosome DNA was obtained directly by HPLC within 30 min after the samples were loaded. Results By Light Cycler we detected the values of the Tm from 24 samples obtained within 10 min. The purity of DNA was not visiblely affect the result. The GC% from HPLC method was stable and accurate, and it was more similar to the published values. But the procedure for further purification of DNA should be included.Conclusion The Light Cycler is approprate for clinical diagnosis of bacteria because. The HPLC method is accurate and reliable for bacterial classification." @default.
- W2371057206 created "2016-06-24" @default.
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- W2371057206 date "2000-01-01" @default.
- W2371057206 modified "2023-09-25" @default.
- W2371057206 title "Light cycler and high pressure liquid chromatography in determing the guanine plus cytosine content of bacterial chromosome DNA" @default.
- W2371057206 hasPublicationYear "2000" @default.
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