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- W2371067468 abstract "Based on the technology of protein separation with immobilized metal ion affinity chromatography(IMAC),a method for immobilization of pepsin had been selected.Cu(Ⅱ) chelating chitosan magnetic microspheres were used as carrier immobilizing pepsin.The effects of immobilized time,pH value,enzyme amount on the immobilization were investigated.The optimum immobilization conditions of enzyme were as follows: immobilized time 1.0 h,pH value 4~5,enzyme amount 150 mg·(g carrier)-1.The enzymatic properties and catalysis characteristics of the immobilized pepsin had been studied.Compared with free pepsin,the results were satisfactory." @default.
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- W2371067468 date "2007-01-01" @default.
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- W2371067468 title "Preparation and Properties of Immobilized Pepsin on Cu(II) Chelating Chitosan Magnetic Microspheres" @default.
- W2371067468 hasPublicationYear "2007" @default.
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