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- W2371118502 abstract "Acetylcholinesterase is immobilized on chitosan coated glass fibre membrane using glutaraldehyde as cross-linking reagent.The benificial immobilizing conditions are obtained as follows: 1 mL of enzyme solution is prepared with 0.2 mol/L phosphate buffer(pH 8.0) adding 50 μL 150 U/mL AChE solution,50 μL 5%(V/V) glutaraldehyde and 100 μL 1%(W/W) BSA.The chitosan coated glass fibre membrane is immersed in the enzyme solution for 8 h at 4 ℃.The optimum temperature and pH value of immobilized AChE are 37 ℃and 8.0,and the immobilized AChE could be reused four times." @default.
- W2371118502 created "2016-06-24" @default.
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- W2371118502 date "2013-01-01" @default.
- W2371118502 modified "2023-09-23" @default.
- W2371118502 title "Immobilization of Acetylcholinesterase with Chitosan Coated Glass Fibre Membrane" @default.
- W2371118502 hasPublicationYear "2013" @default.
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