Matches in SemOpenAlex for { <https://semopenalex.org/work/W2371146088> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2371146088 abstract "Objective:To obtain the more purified astrocytes to settle background for further study.Methods:The cortical tissue of SD rat brain isolated was digested with 0.125% trypsin and cultured with DMEM/F12 medium containing 20% blood serum. Growth condition of the cells was observed under reversed microscope every day. The derivation of the cells was identified by immunohistochemical staining with anti-mouse GFAP.Result:Almost pure cells were obtained after primary culture and subculture. The cell showed unregulated shapes, big cell body, abundant cytoplasm, many longer apophyses and round or ovi-round karyons which leaned to one side. It coincided with the form of astrocytes. Immunohistochemical staining demonstrated that nearly all of the cells were positive.Conclusion:More purified astrocytes can be obtained by this method." @default.
- W2371146088 created "2016-06-24" @default.
- W2371146088 creator A5084915949 @default.
- W2371146088 date "2004-01-01" @default.
- W2371146088 modified "2023-09-25" @default.
- W2371146088 title "Culture and identification of astrocytes from the cortical tissue of new rat brain in vitro" @default.
- W2371146088 hasPublicationYear "2004" @default.
- W2371146088 type Work @default.
- W2371146088 sameAs 2371146088 @default.
- W2371146088 citedByCount "0" @default.
- W2371146088 crossrefType "journal-article" @default.
- W2371146088 hasAuthorship W2371146088A5084915949 @default.
- W2371146088 hasConcept C105702510 @default.
- W2371146088 hasConcept C142724271 @default.
- W2371146088 hasConcept C1491633281 @default.
- W2371146088 hasConcept C153911025 @default.
- W2371146088 hasConcept C169760540 @default.
- W2371146088 hasConcept C181199279 @default.
- W2371146088 hasConcept C190062978 @default.
- W2371146088 hasConcept C202751555 @default.
- W2371146088 hasConcept C203014093 @default.
- W2371146088 hasConcept C204232928 @default.
- W2371146088 hasConcept C2777542381 @default.
- W2371146088 hasConcept C2780340462 @default.
- W2371146088 hasConcept C529278444 @default.
- W2371146088 hasConcept C54355233 @default.
- W2371146088 hasConcept C55493867 @default.
- W2371146088 hasConcept C71924100 @default.
- W2371146088 hasConcept C74864618 @default.
- W2371146088 hasConcept C81885089 @default.
- W2371146088 hasConcept C86803240 @default.
- W2371146088 hasConcept C87863119 @default.
- W2371146088 hasConcept C89423630 @default.
- W2371146088 hasConcept C90703135 @default.
- W2371146088 hasConcept C95444343 @default.
- W2371146088 hasConceptScore W2371146088C105702510 @default.
- W2371146088 hasConceptScore W2371146088C142724271 @default.
- W2371146088 hasConceptScore W2371146088C1491633281 @default.
- W2371146088 hasConceptScore W2371146088C153911025 @default.
- W2371146088 hasConceptScore W2371146088C169760540 @default.
- W2371146088 hasConceptScore W2371146088C181199279 @default.
- W2371146088 hasConceptScore W2371146088C190062978 @default.
- W2371146088 hasConceptScore W2371146088C202751555 @default.
- W2371146088 hasConceptScore W2371146088C203014093 @default.
- W2371146088 hasConceptScore W2371146088C204232928 @default.
- W2371146088 hasConceptScore W2371146088C2777542381 @default.
- W2371146088 hasConceptScore W2371146088C2780340462 @default.
- W2371146088 hasConceptScore W2371146088C529278444 @default.
- W2371146088 hasConceptScore W2371146088C54355233 @default.
- W2371146088 hasConceptScore W2371146088C55493867 @default.
- W2371146088 hasConceptScore W2371146088C71924100 @default.
- W2371146088 hasConceptScore W2371146088C74864618 @default.
- W2371146088 hasConceptScore W2371146088C81885089 @default.
- W2371146088 hasConceptScore W2371146088C86803240 @default.
- W2371146088 hasConceptScore W2371146088C87863119 @default.
- W2371146088 hasConceptScore W2371146088C89423630 @default.
- W2371146088 hasConceptScore W2371146088C90703135 @default.
- W2371146088 hasConceptScore W2371146088C95444343 @default.
- W2371146088 hasLocation W23711460881 @default.
- W2371146088 hasOpenAccess W2371146088 @default.
- W2371146088 hasPrimaryLocation W23711460881 @default.
- W2371146088 hasRelatedWork W1965376606 @default.
- W2371146088 hasRelatedWork W1986349473 @default.
- W2371146088 hasRelatedWork W2009345585 @default.
- W2371146088 hasRelatedWork W2009828521 @default.
- W2371146088 hasRelatedWork W2011704181 @default.
- W2371146088 hasRelatedWork W2022231093 @default.
- W2371146088 hasRelatedWork W2029026888 @default.
- W2371146088 hasRelatedWork W2038248402 @default.
- W2371146088 hasRelatedWork W2055810959 @default.
- W2371146088 hasRelatedWork W2103859975 @default.
- W2371146088 hasRelatedWork W2105302286 @default.
- W2371146088 hasRelatedWork W2116779449 @default.
- W2371146088 hasRelatedWork W2149351216 @default.
- W2371146088 hasRelatedWork W2354936177 @default.
- W2371146088 hasRelatedWork W2356339878 @default.
- W2371146088 hasRelatedWork W2356919404 @default.
- W2371146088 hasRelatedWork W2368684457 @default.
- W2371146088 hasRelatedWork W2890794262 @default.
- W2371146088 hasRelatedWork W75182925 @default.
- W2371146088 hasRelatedWork W1971105892 @default.
- W2371146088 isParatext "false" @default.
- W2371146088 isRetracted "false" @default.
- W2371146088 magId "2371146088" @default.
- W2371146088 workType "article" @default.