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- W2371148614 abstract "The feasibility and the efficiency of excision of marker genes in transgenic rice were studied at transformed cell level by using the Cre/loxP system.The vector pNCG including estrogen-induced Cre/loxP marker gene excision system was introduced to rice cell via Agrobacterium-mediated transformation.After the resistant calli were screened out under G418 selection,the Cre gene expression was induced to excise the marker gene by adding 25 μmol/L estrogen to the medium at different stages of tissue culture.The integration of marker gene nptⅡ,recombinase gene Cre and target gene gusA in regenerated transgenic rice plants was analyzed by PCR.The plantlet of gusA(+)/nptⅡ(-)/Cre(-) in PCR test were calculated as transgenic plants with excision successful.Results showed that marker gene sequences could be excised successfully by estrogen induction at three tissue culture stages including before pre-differentiation,pre-differentiation and differentiation.The frequency of marker gene excision ranged from 6.82% to 46.43%.When transformed calli were treated by estrogen in liquid medium for 3 d before pre-differentiation,the efficiency of marker gene excision in T0 transgenic rice plants was as high as 173.33%,mainly because the estrogen treatment improved the green plant regeneration frequency of calli.When estrogen was directly added to differentiation medium for induction treatment,the frequency of marker gene excision in T0 transgenic rice plants was 46.43% with a higher efficiency of marker gene excision(144.44%).The results confirmed that marker gene sequences in transgenic rice plants could be excised efficiently and quickly by using the Cre/loxP system under estrogen-induced treatment at resistant calli level." @default.
- W2371148614 created "2016-06-24" @default.
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- W2371148614 date "2011-01-01" @default.
- W2371148614 modified "2023-09-24" @default.
- W2371148614 title "Excision of Marker Genes in Transgenic Rice at Transformed Cell Level using Cre/loxP System" @default.
- W2371148614 hasPublicationYear "2011" @default.
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