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- W2371154589 abstract "Objective To establish a HPLC method for determination of indapamide in human whole blood.Methods Chromatographic separation was achieved on a Eurospher-100C C18 analytical column.The column temperature was set at 20℃,the mobile phase was composed of phosphate buffer(pH 3.0)∶ methanol∶ isopropyl alcohol(54∶ 40∶ 6)at a flow rate of 1ml/min,and the UV detection wavelength was set at 241nm.The external standard method was used for the analysis.Results The linearity of the method was good within the range of 5.0~160.0 ng/ml,r=0.9984.The lowest detection limit was 5.0 ng/ml,within-day and between-day RSD were less than 6%,the average recovery was over 70%,and the accuracy was within 100.8%~106.3%.Conclusion The established HPLC method is simple rapid and can be used for the study of bioavailability and bioequivalence of indapamide." @default.
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- W2371154589 date "2011-01-01" @default.
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- W2371154589 title "Determination of Indapamide in Human Whole Blood by HPLC" @default.
- W2371154589 hasPublicationYear "2011" @default.
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