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- W2371183219 abstract "Objective:To prepare the polyclonal antibody detecting sugarcane mosaic virus strain E(ScMV-E).Methods:The prokaryotic expression plasmid was constructed by ligating the target coat protein(CP) gene of ScMV-E into the vector pET29a(+).Through detection by PCR,digestion and sequencing,one of the positive clones was named as pET29a-CP and used for the following experiment.After SDS-PAGE analysis,the recombinant CP of ScMV-E purified by His Trap Kit was injected into rabbits,the corresponding polyclonal antibody specific to ScMV-E was obtained.Then SAP-ELISA,Western blot and tissue blot-ELISA was applied in the detection of its speciality.Results:The result of SDS-PAGE anal-ysis showed that specific expression of a 43 kD fusion protein with 6 His trap was achieved.Western blot analysis con-firmed the fact that the CP of ScMV-E was specifically expressed.Titer of ScMV-E antibody was 1:81 920 by SAP-ELISA.Tissue blot-ELISA analysis of sugarcane leaves collected in the field showed that the ScMV-E antibody can be used directly in the detection of ScMV-E.Conclusion:The polyclonal antibody obtained here could be used to detect ScMV-E directly or to prepare corresponding kit." @default.
- W2371183219 created "2016-06-24" @default.
- W2371183219 creator A5009055298 @default.
- W2371183219 date "2009-01-01" @default.
- W2371183219 modified "2023-09-25" @default.
- W2371183219 title "Purification of Recombinant Coat Protein of Sugarcane Mosaic Virus Strain E and Preparation of its Polyclonal Antibody" @default.
- W2371183219 hasPublicationYear "2009" @default.
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