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- W2371184696 abstract "Objective To clone the TS498 gene of breast cancer MCF-7 cells-associated Trichinella spiralis and express in prokaryotic cells.Methods TS498 gene was amplified from phage library by PCR and subcloned into prokaryotic expression vector pET-28a(+).The constructed recombinant plasmid pET-28a-TS498 was transformed to E.coli BL21(DE3) and induced with IPTG.The expressed product was identified by SDS-PAGE and Western blot.Results The homology of amplified TS498 gene sequence was 100% to that reported in GenBank.Restriction analysis and PCR proved that recombinant plasmid pET-28a-TS498 was constructed correctly.The expressed recombinant protein,with a relative molecular mass of about 23 000,contained about 20% of total somatic protein and was recognized by rabbit antiserum against muscle larvae of T.spiralis.Conclusion The TS498 gene of MCF-7 cells-associated T.spiralis was successfully cloned and expressed in E.coli,which laid a foundation of further study on the character and function of the gene." @default.
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- W2371184696 date "2012-01-01" @default.
- W2371184696 modified "2023-09-24" @default.
- W2371184696 title "Cloning and expression of TS498 gene of breast cancer MCF-7 cells-associated Trichinella spiralis" @default.
- W2371184696 hasPublicationYear "2012" @default.
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