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- W2371190958 abstract "Objective:To study the effects of anti-oncogene PTEN transfection on apoptosis of human bladder cancer cell lines,BIU-87,and to bring a new method of gene therapy for bladder cancer. Methods:A eukaryotic expression vector containing PTEN was transfected into BIU-87,and positive cell clones were selected and amplified.Expression of PTEN was detected by RT-PCR.Apoptosis of BIU-87 were measured before and after transfection by in situ cell apoptosis detection kit and flow cytometry. Results:PTEN transfected BIU-87 could steadily express PTEN mRNA,and cell apoptosis significantly increased compared with control groups. Conclusion:Transfection of PTEN might induce apoptosis of bladder cancer cells so as to inhibit the occurrence and development of tumor." @default.
- W2371190958 created "2016-06-24" @default.
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- W2371190958 date "2005-01-01" @default.
- W2371190958 modified "2023-09-26" @default.
- W2371190958 title "Effects of PTEN gene transfection on apoptosis of human bladder cancer cell lines BIU-87" @default.
- W2371190958 hasPublicationYear "2005" @default.
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