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- W2371192579 abstract "The cristae ampullaris and maculae acusticae were isolated from the vestibule organs of the guinea pig.They were treated with collagenase for 50 or 60 minutes, then cells were separated mechanically.In each ear, two kind hair cells were obtained and counted at the initial time: 83(±23) cells.Their common characteristics were cuticular plate and cilia.Type I hair cells were flask shape and had a clear neck in the middle part.Type Ⅱ hair cell bodies were round and ellipse.Its body was smaller than the type I one.The propertion of type I to type Ⅱ is 62:19.Under correct conditions about 40% of hair cells could still be maintained in vitro for 5 hours after dissociation.In early stage, cell′s change was granule appearance of the cytoplasm.The experiment result showed that enzymatic and mechanical dissociation could be used to obtain a large of single and living hair cells and to investigate the physiology of mammalian vestibular cells." @default.
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- W2371192579 date "1997-01-01" @default.
- W2371192579 modified "2023-09-25" @default.
- W2371192579 title "Isolation of Single Hair Cells in the Vestibule of the Guinea Pig" @default.
- W2371192579 hasPublicationYear "1997" @default.
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