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- W2371246223 abstract "Five kinds of bacteria were isolated from disease Meretrix meretrix and culture water (two iso-lated from the M. meretrix, three from the culture water) in Lvsi harbor, Jiangsu province. The results of in-fection showed that strain WG1702 can cause disease with the mortality rate 100 percent and the same symptoms as original. It was cued that strain WG1702 was the pathogenic bacteria and caused the death in large scare, a preliminary research was done on the bacteria including morphological, physiological, bio-chemical characteristics, phylogenetic position and pathogenic. Strain WG1702 was a straight-rod gram negative bacterium, the physiological and biochemical indicators were lysine decarboxylase, ornithine de-carboxylase, reduction of NO3? to NO2?, mannitose, oxidase and methylred reaction positive, arginine de-carboxylase, arginine dihydrolase, salicin and voges-proskauer negative. In order to identify the bacteria phylogenetic position and a 16S rRNA molecular identification was done, PCR amplification got a size of about 1.5 kb gene fragment, The whole 16S rRNA sequences were determined, analyzed the homology of sequences and built the phylogenetic tree. Sequences analysis revealed that strain WG1702 exhibited the highest level of 97.4% similarity to Vibrio harveyi(DQ146937), combining morphological, physiological and biochemical identification results, the isolated strain WG1702 was identified as Vibrio harveyi." @default.
- W2371246223 created "2016-06-24" @default.
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- W2371246223 date "2009-01-01" @default.
- W2371246223 modified "2023-09-25" @default.
- W2371246223 title "Pathogens Isolation and Identification of Eruptive Epidemic Disease in Meretrix meretrix" @default.
- W2371246223 hasPublicationYear "2009" @default.
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