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- W2371256265 abstract "Objective To study the effect of vascular endothelial growth factor(VEGF) transfected rat vascular endothelial cell on angiogenesis with enhanced green fluorescent protein(EGFP) carrier and transplanted rat in vivo,and to observe neovessels by its inducing for providing basis of transplanting tissue inducing neovessels.Methods The vascular endothelial cell was transfected by pIRES2-EGFP plasmid,and then transplanted it under the capsule of kidney.The EGFP expression was observed by fluorescence microscope.Flurocytometery were applied to evaluate the transfection efficiency.The VEGF mRNA expression was detected by RT-PCR.Immunohistochemical staining of human VEGF165 was used to determine the expression of VEGF in vessel endothelial cells.The histology and angiogenesis of kidney were studied with HE staining.Results There was specific EGFP expression in epithelial cells observed by fluromicroscope.The transfected ratio was 13.06 % determined by flurocytometery.In experimental group the VEGF expression was found in vascular cell nucleus and cytoplasm.The human orgin VEGF165 gene in vascular endothelial cells of experimental rat expressed at mRNA level was shown by RT-PCR.Fourteen days after transplantation,the neocapillary net angionenesis was found under the kidney capsule in experimental rat.Although the vascular endothelial cells were still alive,but markable blood sinus was not formed.Conclusion The transfection of VEGF is an effective approach for promoting endothelial cells to form neovessels in early stage(within 14 days)." @default.
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- W2371256265 date "2007-01-01" @default.
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- W2371256265 title "Effect of VEGF_(165) transfected rat vascular endothelial cell on angiogenesis" @default.
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