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- W2371279114 abstract "Objective To sumcnarize the expression profile of transitional cell carcinoma by shotgun proteome strategy,and based on which the tumor-stroma interaction and biomarker panel discovery from urine were discussed.Methods Specimens of 4 bladder transitional cell carcinoma were prespectively collected.Cancer cells and stroma were purified by laser capture microdissection(LCM).Peptide mixture was separated by two-dimensional liquid chromatography(2D-LC) followed by electrospray ionization-tandem mass spectrometry(ESI-MS/MS) identification.And then bioinformatics of the identified proteins were analyzed and compared with the published literature.Results A total of 775 proteins commonly expressed in the 4 specimens,612 of which had biological process annotation as to the Gene Ontology(GO).There were 40/25 GO terms exhibited as enriched/depleted within the biological process annotation.The enriched GO terms centered on metabolism process.117 proteins expressed both in cancer cells and stroma.Among them,73 proteins were located in well-known pathways.Proteins belonging to the term of cell adhesion,cell proliferation,cell differentiation were good candidate biomarkers for cancer detection from urine.Conclusions The present expression profile provids valuable information for the understanding of cell biology,tumor-stroma interaction as well as the discovery of biomarkers from urine." @default.
- W2371279114 created "2016-06-24" @default.
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- W2371279114 date "2011-01-01" @default.
- W2371279114 modified "2023-09-25" @default.
- W2371279114 title "Proteome expression profile of transitional cell carcinoma and biomarker discovery" @default.
- W2371279114 hasPublicationYear "2011" @default.
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