Matches in SemOpenAlex for { <https://semopenalex.org/work/W2371293497> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2371293497 abstract "A pair of degenerating primers was designed according to the conserved regions of β-1,3-glucanase gene from other plant species and a cDNA fragment was isolated from Brassica oleracea L.using RT-PCR.Full-length cDNA with 1 277 bp was acquired by 5′RACE and 3′RACE methods.Its ORF(open read frame)encoded a polypeptide of 352 amino acids.The sequence was accepted and released by GenBank(accession number EF484879)and named as BObg.The putative protein of this gene had an isoelectric point of 7.314 and a calculated molecular weight of 3.892×104.Alignment and phylogenetic tree showed it had high homologies with β-1,3-glucanase gene from other plant species.Semi quantitative RT-PCR analysis indicated this gene was up-regulated expressed at 48 h after downy mildew was inoculated,while it was down-regulated expressed in the plant without inoculation and other phases post inoculation.It was subsequently cloned into plant expression vector pBI121 and located between 35S promoter and NOS(nopaline synthase)terminator." @default.
- W2371293497 created "2016-06-24" @default.
- W2371293497 creator A5016717010 @default.
- W2371293497 date "2008-01-01" @default.
- W2371293497 modified "2023-09-23" @default.
- W2371293497 title "Cloning and characterization of β-1,3-glucanase gene cDNA from Brassica oleracea L. and construction of its plant expression vector" @default.
- W2371293497 hasPublicationYear "2008" @default.
- W2371293497 type Work @default.
- W2371293497 sameAs 2371293497 @default.
- W2371293497 citedByCount "0" @default.
- W2371293497 crossrefType "journal-article" @default.
- W2371293497 hasAuthorship W2371293497A5016717010 @default.
- W2371293497 hasConcept C104317684 @default.
- W2371293497 hasConcept C121050878 @default.
- W2371293497 hasConcept C150194340 @default.
- W2371293497 hasConcept C15190224 @default.
- W2371293497 hasConcept C153911025 @default.
- W2371293497 hasConcept C167625842 @default.
- W2371293497 hasConcept C187882448 @default.
- W2371293497 hasConcept C19924922 @default.
- W2371293497 hasConcept C199360897 @default.
- W2371293497 hasConcept C2777943035 @default.
- W2371293497 hasConcept C2780087670 @default.
- W2371293497 hasConcept C33161422 @default.
- W2371293497 hasConcept C40767141 @default.
- W2371293497 hasConcept C41008148 @default.
- W2371293497 hasConcept C47289529 @default.
- W2371293497 hasConcept C50724178 @default.
- W2371293497 hasConcept C54355233 @default.
- W2371293497 hasConcept C59822182 @default.
- W2371293497 hasConcept C79029880 @default.
- W2371293497 hasConcept C86803240 @default.
- W2371293497 hasConceptScore W2371293497C104317684 @default.
- W2371293497 hasConceptScore W2371293497C121050878 @default.
- W2371293497 hasConceptScore W2371293497C150194340 @default.
- W2371293497 hasConceptScore W2371293497C15190224 @default.
- W2371293497 hasConceptScore W2371293497C153911025 @default.
- W2371293497 hasConceptScore W2371293497C167625842 @default.
- W2371293497 hasConceptScore W2371293497C187882448 @default.
- W2371293497 hasConceptScore W2371293497C19924922 @default.
- W2371293497 hasConceptScore W2371293497C199360897 @default.
- W2371293497 hasConceptScore W2371293497C2777943035 @default.
- W2371293497 hasConceptScore W2371293497C2780087670 @default.
- W2371293497 hasConceptScore W2371293497C33161422 @default.
- W2371293497 hasConceptScore W2371293497C40767141 @default.
- W2371293497 hasConceptScore W2371293497C41008148 @default.
- W2371293497 hasConceptScore W2371293497C47289529 @default.
- W2371293497 hasConceptScore W2371293497C50724178 @default.
- W2371293497 hasConceptScore W2371293497C54355233 @default.
- W2371293497 hasConceptScore W2371293497C59822182 @default.
- W2371293497 hasConceptScore W2371293497C79029880 @default.
- W2371293497 hasConceptScore W2371293497C86803240 @default.
- W2371293497 hasLocation W23712934971 @default.
- W2371293497 hasOpenAccess W2371293497 @default.
- W2371293497 hasPrimaryLocation W23712934971 @default.
- W2371293497 hasRelatedWork W2068735936 @default.
- W2371293497 hasRelatedWork W2071379807 @default.
- W2371293497 hasRelatedWork W2085047733 @default.
- W2371293497 hasRelatedWork W2208584869 @default.
- W2371293497 hasRelatedWork W2283096184 @default.
- W2371293497 hasRelatedWork W2351174605 @default.
- W2371293497 hasRelatedWork W2352305496 @default.
- W2371293497 hasRelatedWork W2358080801 @default.
- W2371293497 hasRelatedWork W2359622651 @default.
- W2371293497 hasRelatedWork W2360911116 @default.
- W2371293497 hasRelatedWork W2362302768 @default.
- W2371293497 hasRelatedWork W2362472977 @default.
- W2371293497 hasRelatedWork W2369498966 @default.
- W2371293497 hasRelatedWork W2376123553 @default.
- W2371293497 hasRelatedWork W2376174789 @default.
- W2371293497 hasRelatedWork W2383377208 @default.
- W2371293497 hasRelatedWork W2389728696 @default.
- W2371293497 hasRelatedWork W2390165566 @default.
- W2371293497 hasRelatedWork W2393497592 @default.
- W2371293497 hasRelatedWork W2815530191 @default.
- W2371293497 isParatext "false" @default.
- W2371293497 isRetracted "false" @default.
- W2371293497 magId "2371293497" @default.
- W2371293497 workType "article" @default.