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- W2371304888 abstract "Objective To establish a sensitive, specific and simple measurement for serum collagen Ⅳ which can be used with HBsAg, HBsAb. HBeAb HBcAg and HbcAb. Method By Preparing anti - human type Ⅳ collagen IgG with rabbits and utilizing sodium periodate horse - radish peroxidase - labelling anti - human type Ⅳ collagen monoclonal antibody to change dilution component and concentration, we established sandwich ELISA. Results ELISA had a lowest testing limit of 30μg/L, the average recovery was 102. 75%, CVs of intra - assay were respectively 6.0 and 11.8% and the reference value by testing 50 healthy people was 97.9μg/L±36.3μg/L, the average value of liver cirrhosis type Ⅳ collagen was 254.82μg/L±69.9μ/g. Conclusion The improved method is sensitive, exact, stable and spe- cific with small amount of sample and short testing time. It is easy to spread in clinical practice." @default.
- W2371304888 created "2016-06-24" @default.
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- W2371304888 date "2001-01-01" @default.
- W2371304888 modified "2023-09-23" @default.
- W2371304888 title "The research on measurement Type IV collagen" @default.
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