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- W2371329088 abstract "Objective:To establish real-time fluorescence quantitative polymerase chain reaction(RFQ-PCR) for measurement of APRIL expression level and its receptors in B-ALL children.Methods:Specific primers and TaqMan probes of APRIL and its receptors were designed,and fluorescence of the PCR products were detected continuously during amplification.According to the standard curves created by plasmid DNA,the expression level of target genes in clinical samples were determined by using software,and the results were presented as the ratios of target genes' mRNA to β2 Microgluobulin(β2M)'s mRNA.Results:The results from 18 samples showed that the expression level of APRIL,BCMA and TACI in the peripheral blood of B-ALL were significantly higher than that in normal children(P0.05).Conclusions:APRIL may play a great role in the development,and progress of B-ALL through its receptor B cell maturation antigen(BCMA) and transmembrane activator and CAML interactor(TACI)." @default.
- W2371329088 created "2016-06-24" @default.
- W2371329088 creator A5060108385 @default.
- W2371329088 date "2010-01-01" @default.
- W2371329088 modified "2023-09-25" @default.
- W2371329088 title "Measurement of the expression level of APRIL and its receptors in B-ALL by real-time fluorenscence quantitative method" @default.
- W2371329088 hasPublicationYear "2010" @default.
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