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- W2371361808 abstract "The viral pathogens are the most important epidemic disease affecting fish,which cause significant morbidity and mortality in fish.It has substantial impact on the production of fish and can not be prevented effectively.In order to determinate which pathogen caused the mass mortality cultured common carp(Cyprinus carpio),a series of experiments about cell lines challenged with virus supernatants from diseased fish homogenate,plaque assays,observed with electron microscope and artificial infection in fish were done.Diseased fish samples were collected,homogenized,filtered,then inoculated onto monolayer cultures of 14 cell lines including Ctenopharyngodon idellus ovaries(GCO),Ctenopharyngodon idellus fins(GCF),Channel catfish ovary(CCO), Epithelioma papulosum cyprini(EPC), Pimephales promelas(FHM),Cyprinus carpio leucocyte cell(CLC),Trionyx sinensis blood(TSB), Carassiu auratus gibelio heart(GCH), Ameiurus nebulosus(BB), Carassius auratus blastula(CAB),Carassius auratus vertebral column bone(CAVB), Ctenopharngodon idellus kidney(CIK), Gobiocypris rarus ovary(GRO) and Hypophthalmichthys molitrix ovary(HMO).The results show that observation of the cytopathogenic effect(CPE) appeared in 9 of these cell lines,GCO,GCF,CCO,EPC,FHM,CLC,TSB,GCH and BB.The CPE involved cell rounding,detachment from the monolayer, and subsequent destruction of monolayer.Virus titers of about 10~(7.3) TCID_(50)/mL were obtained by infecting the GCF cells.Viral plaques were formed after virus supernatants attachment to three cell lines FHM,TSB,and GCO,and the plaques of 1 to 4 mm in diameter is probably dependent on host cell lines and viral diluents.Electron microscopy observation revealed that the viral particles of ultrathin sections and negatively stained had a typical bullet-shape morphology.Initial symptoms appear in crucian carp(Carassius auratus) and common carp(Cyprinus carpio) in 2 and 3 days post-infection(p.i.) respectively.The diseased fish died in 1 to 2 days after the symptoms appearing.The late mortality rate of infected fish was 83.3% until the 14 days p.i.The dying fish were homogenized,filtered,then inoculated onto fish cell lines,the CPE were observed obviously in 24 hours p.i.and similar to those induced by the original homogenate of the diseased common carp.It is supported that the diseased fish were infected by the viral pathogen.Also the viral pathogen has strong infectivity to the crucian carp(Carassius auratus) and common carp(Cyprinus carpio).The detection of viral pathogen from diseased common carp(Cyprinus carpio) by infectious tests in our researches is a base to the isolation and indentification of the viral pathogen,also to the diagnosis and prophylaxis of it." @default.
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- W2371361808 date "2006-01-01" @default.
- W2371361808 modified "2023-10-13" @default.
- W2371361808 title "Detection of viral pathogen from diseased common carp (Cyprinus carpio) by infectious tests" @default.
- W2371361808 hasPublicationYear "2006" @default.
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