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- W2371410319 abstract "Competitive quantitative PCR(cPCR) assay was developed for monitoring and detecting porcine circovirus (PCV-2) DNA. Using PCR assay, the cPCR was based on competitive co-amplification of a 490 bp region of the PCV-20RF2 gene, with a known concentration of competitor DNA, which produced a 692 bp fragment. The cPCR was validated by quantification of a known amount of competitor PCV DNA with one series of dilutions of target DNA. Using the regression equation, we protracted the standard curve and affirmed the quantity of the competitive template. We used this technique to determine PCV genome copy numbers in infected cells. Furthermore, we measured PCV DNA loads in clinical sample and found PCV2 DNA content in the piglet with PMS is higher than 1.0145 × 10~8 copies/ml in lung, 0.8 × 10~7 copies/mL in blood, and 9.698 × 10~8 copies/mL in lymph. Our research might provide some hints to the study of the future work." @default.
- W2371410319 created "2016-06-24" @default.
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- W2371410319 date "2004-01-01" @default.
- W2371410319 modified "2023-09-23" @default.
- W2371410319 title "Development and appfication of a competitive quantitative assay for monitoring porcine circovirus DNA" @default.
- W2371410319 hasPublicationYear "2004" @default.
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