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- W2371435937 abstract "objective: To construct adeno-associated virus vector encoding the SD rat brain derived neurotrophic factor (BDNF) gene and examine its ability to express the BDNF gene in vitro. Methods: The specific BDNF sequence was cloned into the plasmid of pAAV-MCS in AAV helper-Free system to construct the BDNF expression plasmid pAAV-MCS-BDNF. The recombinant plasmids were identified by DNA sequencing and restriction digestion. Then the packaging cell lines (HEK293 cell) were cotransfected with the pAAV-MCS-BDNF together with the control plasmid pAAV-RV and pHelper by phosphate-calcium deposit method. The recombinant adeno-associated virus vector infected the hela cell, the expression of BDNF was detected by Western blot, RT-PCR and ELISA method, Results: The recombinant adeno-associated virus vector carrying the BDNF Sequence was constructed successfully, the viral titer was 1.29×108 and it could up-regulated the expression of the BDNF gene following with the time. Conclusions: The recombinant adeno-associated virus vector rAAV-BDNF can enhance the expression of BDNF gene significantly, which lays the basis for its application in the treatment of the neurodamaged disease." @default.
- W2371435937 created "2016-06-24" @default.
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- W2371435937 date "2007-01-01" @default.
- W2371435937 modified "2023-09-27" @default.
- W2371435937 title "Construction and identification of recombinant adeno-associated virus vector encoding BDNF by Helper-Free system" @default.
- W2371435937 hasPublicationYear "2007" @default.
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