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- W2371448175 abstract "Objective To investigate the influence of the compositions of human breast milk on the quantitative detection of HBV-DNA and the distribution of HBV-DNA in the human breast milk, and to provide the basis to set up a feasible and standard method for the quantitative detection of HBV-DNA in the human breast milk. Methods We set up the models interfere with cheese, whey, sediment and the compared model of physiological saline, and contrasted the HBV-DNA quantitative detection of interferential-model with compared-model. Then we mixed the high-value, mid-value and low-value standard HBV-DNA positive serum with HBV-DNA negative breast milk, and detected the HBV-DNA load in the cheese, whey and sediment. Results Contrasted the HBV-DNA quantitative detection of the cheese interferential-model, whey interferential-model, sediment interferential-model with the physiological saline compared-model, the value of t were 3.30, 3.00 and 4.60, and the value of P were less than 0.05. Contrasted the HBV-DNA quantitative detection between the cheese interferential-model, whey interferential-model and sediment interferential-model, the value of P were greater than 0.05. In the condition of mixed negative breast milk with high-value and mid-value standard serum, contrasted the HBV-DNA quantitative detection between the cheese, whey and sediment, the value of P were less than 0.05, and the quantity of HBV-DNA in the sediment was highest, in the whey was secondly and in the cheese was lowest. In the condition of mixed negative breast milk with low-value standard serum, the value of P were greater than 0.05 except the value of P contrasted between cheese and sediment was less than 0.05. Conclusion The compositions of human breast milk can interfere the quantitative detect of HBV-DNA, which makes the estimated value less than the actual value. The HBV-DNA mostly exists in the sediment, also in the cheese and whey." @default.
- W2371448175 created "2016-06-24" @default.
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- W2371448175 date "2013-01-01" @default.
- W2371448175 modified "2023-09-28" @default.
- W2371448175 title "Influence of human breast milk on the quantitative detection of HBV-DNA" @default.
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