Matches in SemOpenAlex for { <https://semopenalex.org/work/W2371497989> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2371497989 abstract "AIM: To investigate the cellular mechanisms by which PGF_ 2α promotes glucose-stimulated insulin secretion in NIT-1 beta cells. METHODS: Using the radioimmunoassay (RIA), the amount of the PGF_ 2α augmentation of glucose stimulated insulin secession was determined in different conditions, and the confocal laser scanning methods by Fluo-3AM as a fluorescent probe were used to analyze the changes of intracellular calcium in NIT-1β cells. RESULTS: At the lower glucose (0, 5.5 mmol/L), PGF_ 2α (5 μmol/L) failed to potentiate insulin secretion (P0.05). However, in the presence of 16.5 mmol/L glucose, PGF_ 2α increased significantly in insulin secretion (P0.05). Neither the AC inhibitor ddA nor the GC inhibitor Ly-83583 altered PGF_ 2α-potentiated insulin secretion in the presence of 16.5 mmol/L (P0.05 or P0.01). Otherwise, the PLC inhibitor U-73122 and the PKC blocker calphostin C both counteracted the insulinotropic of PGF_ 2α (P0.01 or P0.05). Moreover, exposure of the NIT-1β cells to 5 μmol/L PGF_ 2α induced a rapid increase of intracellular calcium (P0.01). The inhibitor, ddA or Ly-83583 had no impact on PGF_ 2α-induced elevation of the intracellular calcium (P0.01). Pretreatment of the cells with U-73122 completely prevented the calcium response induced by PGF_ 2α (P0.01). CONCLUSION: Efects of PGF_ 2α was independent of cAMP or cGMP, potentiated glucose (16.5 mmol/L)-induced insulin secretion in NIT-1β cells through stimulation of phospholipase C, which subsequently mediated the elevation of intracellular calcium and activation of protein kinase C." @default.
- W2371497989 created "2016-06-24" @default.
- W2371497989 creator A5089333862 @default.
- W2371497989 date "2006-01-01" @default.
- W2371497989 modified "2023-09-25" @default.
- W2371497989 title "Mechanisms of PGF_ (2α) on the glucose-induced insulin secretion in NIT-1β cells" @default.
- W2371497989 hasPublicationYear "2006" @default.
- W2371497989 type Work @default.
- W2371497989 sameAs 2371497989 @default.
- W2371497989 citedByCount "0" @default.
- W2371497989 crossrefType "journal-article" @default.
- W2371497989 hasAuthorship W2371497989A5089333862 @default.
- W2371497989 hasConcept C126322002 @default.
- W2371497989 hasConcept C134018914 @default.
- W2371497989 hasConcept C170493617 @default.
- W2371497989 hasConcept C176685330 @default.
- W2371497989 hasConcept C181080969 @default.
- W2371497989 hasConcept C185592680 @default.
- W2371497989 hasConcept C195794163 @default.
- W2371497989 hasConcept C2778597717 @default.
- W2371497989 hasConcept C2779306644 @default.
- W2371497989 hasConcept C2780245509 @default.
- W2371497989 hasConcept C49039625 @default.
- W2371497989 hasConcept C519063684 @default.
- W2371497989 hasConcept C55493867 @default.
- W2371497989 hasConcept C62478195 @default.
- W2371497989 hasConcept C71924100 @default.
- W2371497989 hasConcept C79879829 @default.
- W2371497989 hasConcept C86803240 @default.
- W2371497989 hasConceptScore W2371497989C126322002 @default.
- W2371497989 hasConceptScore W2371497989C134018914 @default.
- W2371497989 hasConceptScore W2371497989C170493617 @default.
- W2371497989 hasConceptScore W2371497989C176685330 @default.
- W2371497989 hasConceptScore W2371497989C181080969 @default.
- W2371497989 hasConceptScore W2371497989C185592680 @default.
- W2371497989 hasConceptScore W2371497989C195794163 @default.
- W2371497989 hasConceptScore W2371497989C2778597717 @default.
- W2371497989 hasConceptScore W2371497989C2779306644 @default.
- W2371497989 hasConceptScore W2371497989C2780245509 @default.
- W2371497989 hasConceptScore W2371497989C49039625 @default.
- W2371497989 hasConceptScore W2371497989C519063684 @default.
- W2371497989 hasConceptScore W2371497989C55493867 @default.
- W2371497989 hasConceptScore W2371497989C62478195 @default.
- W2371497989 hasConceptScore W2371497989C71924100 @default.
- W2371497989 hasConceptScore W2371497989C79879829 @default.
- W2371497989 hasConceptScore W2371497989C86803240 @default.
- W2371497989 hasLocation W23714979891 @default.
- W2371497989 hasOpenAccess W2371497989 @default.
- W2371497989 hasPrimaryLocation W23714979891 @default.
- W2371497989 hasRelatedWork W1556056052 @default.
- W2371497989 hasRelatedWork W1969262333 @default.
- W2371497989 hasRelatedWork W1970929172 @default.
- W2371497989 hasRelatedWork W2016188339 @default.
- W2371497989 hasRelatedWork W2030907815 @default.
- W2371497989 hasRelatedWork W2032352600 @default.
- W2371497989 hasRelatedWork W2052134057 @default.
- W2371497989 hasRelatedWork W2053326059 @default.
- W2371497989 hasRelatedWork W2077128471 @default.
- W2371497989 hasRelatedWork W2078312203 @default.
- W2371497989 hasRelatedWork W2092256405 @default.
- W2371497989 hasRelatedWork W2095475365 @default.
- W2371497989 hasRelatedWork W2122603966 @default.
- W2371497989 hasRelatedWork W2124363835 @default.
- W2371497989 hasRelatedWork W2321855881 @default.
- W2371497989 hasRelatedWork W2328575328 @default.
- W2371497989 hasRelatedWork W2350657108 @default.
- W2371497989 hasRelatedWork W2364008945 @default.
- W2371497989 hasRelatedWork W2408329465 @default.
- W2371497989 hasRelatedWork W2805991815 @default.
- W2371497989 isParatext "false" @default.
- W2371497989 isRetracted "false" @default.
- W2371497989 magId "2371497989" @default.
- W2371497989 workType "article" @default.