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- W2371562977 abstract "Objective To study the effect of varics antigen repairing methods on the results of immunohistochemistry staining.(Methods)With Elivision immunohistochemistry method,experiments were conducted on ten deferent kinds of monoclonal antibody under the condition of seven deferent antigen repairing,and same experimental environment and time.Results Satisfied expression effects were shown when ER,PR,CD3,Bc1-2 in pH9.0Tris-EDTA heat repairing,C-erbB-2,PSA,CD117 in citrate buffer heat repairing,and CK in 0.125% of trypsin repairing solution.After being repaired,CD34 was over expressed.The expression effects of ER,PR,CD3,Bc1-2,C-erbB-2,PSA,CD117 in water heat repairing method were superior to those in microwave heat repairing method.Conclusion The quality of immunohistochemistry staining is affected by deferent antigen repairing method as well as the pH of repairing solutions.It can significantly improve the positive rate and accuracy of immunohistochemistry staining results to master the best antibody repairing method." @default.
- W2371562977 created "2016-06-24" @default.
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- W2371562977 date "2006-01-01" @default.
- W2371562977 modified "2023-09-24" @default.
- W2371562977 title "The effect of varics antigen repairing methods on the results of immunohistochemistry staining" @default.
- W2371562977 hasPublicationYear "2006" @default.
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