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- W2371573821 abstract "Objective:To study the prokaryotic expression and function of GRP78 tagged with green fluorescence protein. Methods:The enhanced green fluorescence protein was linked with the C-terminal end of GRP78 so as to track the expression of GRP78. Moreover,the tagged protein conjugated with splenocytes was determined by FACS. Results:The molecular weight of fusion protein GRP-EGFP was 126 kD and the prokaryotic expression was correctly judged by Western Blot. The bacteria BL21 expressing GRP78-EGFP could emit sparklingly green fluorescence excited by ultraviolet light. Compared with the protein of EGFP,GRP78-EGFP was mainly conjugated with neutrophils,and the average binding rate was 5.15%. Conclusion:GRP78 tagged with green fluorescence protein expressed in prokaryotic bacteria retained native conformation and played an important role in trailing interested protein." @default.
- W2371573821 created "2016-06-24" @default.
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- W2371573821 date "2009-01-01" @default.
- W2371573821 modified "2023-09-23" @default.
- W2371573821 title "The study of prokaryotic expression and function of GRP78 tagged with green fluorescence protein" @default.
- W2371573821 hasPublicationYear "2009" @default.
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