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- W2371593540 abstract "A genetic transformation system of embryogenic callus(Saussurea involucrata) mediated by Agrobacterium tumefaciens was established for GFP(green fluorescent protein) gene.The result showed that efficient transformation was obtained with embryogenic callus as acceptor,kanamycin of 40 mg/L was used to screen embryogenic calli.Engineering bacteria witch contain 100 μmol/L AS and 500 mg/L proline infected for 25 min after embryogenic calli was pre-cultured 2 d.Eight resistant plants were to confirm by PCR and detection of GFP fluorescent protein and 6-positive plants obtained,which indicated that GFP gene had integrated into the Saussurea involucrata genome and expressed." @default.
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- W2371593540 date "2010-01-01" @default.
- W2371593540 modified "2023-09-23" @default.
- W2371593540 title "Establishment of Agrobacterium-mediated Transformation System in Saussurea involucrata" @default.
- W2371593540 hasPublicationYear "2010" @default.
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