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- W2371682545 abstract "Taking young branches of Populus alba×P. berolinensis as plant material, effects of pre-cultures, cytokinin/auxin ratio on in vitro regeneration were investigated. Results showed that pre-cultured in MS liquid medium for 3 d was the optimal method. The most suitable bud induction medium was MS+TDZ 0.1 mg/L+NAA 0.02 mg/L, in which multiple buds could directly form. The optimal rooting medium was 1/2 MS+NAA 0.05 mg/L+IBA 0.01 mg/L+ active carbon 0.6 g/L.Moreover, Gt5GT7 gene was transformed into regenerative leaves mediated by Agrobacterium rhizogenesis. GUS staining illustrated that transformation system was successfully established. Moreover, the optimal A600 was 0.45-0.6." @default.
- W2371682545 created "2016-06-24" @default.
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- W2371682545 date "2014-01-01" @default.
- W2371682545 modified "2023-09-24" @default.
- W2371682545 title "Leaf in vitro regeneration and Gt5GT7 genetic transformation in Populus alba×P. berolinensis" @default.
- W2371682545 hasPublicationYear "2014" @default.
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