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- W2371710423 abstract "Objective To prepare the bFGF、 VEGF- impregnated microsphere and to investigate its bioactivity and its effect on endothelials. Methods The bFGF、 VEGF - impregnated microsphere was prepared by emulsified cold -condensation method and was added to the RPMI 1640 medium. The proliferation of cultured endothelials were measured by cell counting and MTT method. Results The average diameter of microsphere was(11.32±3.64)μm; the in vitro cellular study showed no significant difference in the cell number and cell viability of 4 groups 1 day after plate culture. The cell number and cell viability in bFGF、VEGF - impregnated microsphere group were more than those in other groups 5 days after plate culture. 7days after plate culture,the cell number and cell viability in bFGF、 VEGF - impregnated microsphere group were still higher. Conclusion The preparing method is simple and accurate , the bFGF、 VEGF- impregnated microsphere can promote the proliferation of endothelials through a long period of bFGF、 VEGF sustained - release." @default.
- W2371710423 created "2016-06-24" @default.
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- W2371710423 date "2007-01-01" @default.
- W2371710423 modified "2023-09-23" @default.
- W2371710423 title "The preparation of growth factors-impregnated microsphere and its effect on endothelial cells" @default.
- W2371710423 hasPublicationYear "2007" @default.
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