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- W2371727306 abstract "Objective To explore the regularity, efficiency, requirements for long-term survival of human bone marrow mesenchymal stem cells (hMSCs) transforming to neuron induced by 2-mercaptoethanol (2-ME) in vitro. Methods At 6 hours after hMSCs induced in vitro by 2-ME in serum-free medium, induction media were removed and transferred to neuronal induction maintenance media for 6 days. The expressions of specific markers of neuron or astrocyte were evaluated by cytochemistry, immunohistochemistry staining and Western blot method. Results After induction for 6 hours, hMSCs displayed neuronal morphologies such as pyramidal cell bodies. The induced hMSCs expressed neuro-specific marker such as neuro-specific enolase (NSE) and neurofilament (NF), but could not express glia fiber acid protein (GFAP). The number of positive cells of NSE and NF-M was more than 80%. The results of Western blot showed that nestin was detectable at 6h, and expression levels of hMSCs-derived neurons decreased with time by Western blot. With the times, there was no detectable nestin expression in any hMSCs-derived neurons, but MAP-2, a marker of mature neuron was detectable. Conclusions hMSCs could differentiate into nerve stem cells in vitro, and then induced neurocyte could be gradually mature." @default.
- W2371727306 created "2016-06-24" @default.
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- W2371727306 date "2005-01-01" @default.
- W2371727306 modified "2023-09-25" @default.
- W2371727306 title "Experimental study on neuro-differentiation of human bone marrow mesenchymal stem cells in vitro" @default.
- W2371727306 hasPublicationYear "2005" @default.
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