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- W2371781750 abstract "Objective To isolate and cultivate mesenchymal stem cells from human placenta tissue,and investigate the effect of MSCs from human placenta tissue on allogeneic T lymphocyte proliferation. Methods Firstly we isolated human placenta-derived mesenchymal stem cells(PMSCs) from placenta tissue,observed their morphology and identified cell surface antigen expression by FACS analysis and their differentiation potency in vitro. The PMSCs were cultivated and expanded in vitro,then added to two-way mixed lymphocyte reaction(MLR) essay,according to different ratio of PMSCs to lymphocytes. T lymphocyte proliferation is measured after 6 days' coculture. Results MSCs isolated from placenta tissue had the similar morphology and cell surface markers with bone marrow mesenchymal stem cells(BMSCs). CD29,CD44 and CD105 expressions were found on PMSCs but CD34,CD45,CD106 and HLA-DR were not found. PMSCs also can be induced into neuron-like cells. In MLR essay,PMSCs can inhibit T lymphocytes proliferation in vitro.Conclusion The human placenta tissue is proven to be a useful source of the MSCs. PMSCs had the similar biological character and immuno-regulatory effect with BMSC,which will provide alternative cell source for the mesenchymal stem cells." @default.
- W2371781750 created "2016-06-24" @default.
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- W2371781750 date "2009-01-01" @default.
- W2371781750 modified "2023-09-24" @default.
- W2371781750 title "The Effect of Placenta-derived Mesenchymal Stem Cells on T Lymphocytes Proliferation in Vitro" @default.
- W2371781750 hasPublicationYear "2009" @default.
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