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- W2371783856 abstract "Objective:To investigate the effects of icariin(ICA)on proliferation of huaman periodontal ligament cells(human periodontal ligament cells,hPDLC)and the alkaline phosphatase(alkaline phosphatase,ALP)activity inhibited by LPS.Method:HPDLC were cultured in virto and stimulated with icariin with different concentrations(0、10-5、10-6、10-7、10-8、 10-9 mol/L))for 96 hours.The ability of proliferation of HPDLC was detected by MTT method.The activity of alkaline phosphatase and the expression of alkaline phosphatase mRNA was seperately determined by p-Nitrophnyl phosphate(pNPP) method and RT-PCR after being treated with icariin at the concentration of 10-6 mol/L inhibited by LPS.Result:icariin had a dose-dependent effect on the proliferation the hPDLC in a suitable concentration range from 10-6～10-8 mol/L.The alkaline phosphatase activity was remarkably inhibited by incubation of PDLC with 10 μg/mL LPS and was improved in the presence of icariin at the concentration of 10-6 mol/L.Conclusion:Icariin have the effect on the proliferation of PDLC and improve the ALP activity inhibited by LPS,which could help for Peri-apical tissue regeneration." @default.
- W2371783856 created "2016-06-24" @default.
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- W2371783856 date "2013-01-01" @default.
- W2371783856 modified "2023-09-24" @default.
- W2371783856 title "Effects icariin of on the alkline phosphatase activity of human periodontal ligament cells inhibited by LPS" @default.
- W2371783856 hasPublicationYear "2013" @default.
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