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- W2371789098 abstract "Dummy-template molecularly imprinted polymers were synthesized for selective solid-phase extraction of Zidovudine in human serum.Best functional monomer,MAA,and cross-linker,DVB were selected by computer modeling.The MIP for AZT was prepared using self-made dummy template(AZT-ES) and with MAA and DVB in acetonitrile.Solid phase extraction was carried out utilizing the MIP particles as sorbents.After optimization of SPE profile,the best imprinting effect was obtained with loading in 1 mL of K-phosphate buffer pH7,washing with 1 mL of 2%(V/V)acetonitrile/phosphate buffer,eluting with hydrochloric acid:methanol=10:90.MIP and NIP showed high binding capacity in the buffer in breakthrough curve,and their capacities were 9.10 mg and 1.77 mg of drug/1g of adsorbent respectively.The dummy-template molecularly imprinted polymers were tested with zidovudine ester and lamivudine were tested by adsorption experiment,and the results showed high selectivity for AZT.The SPE eluent of AZT in spiked human serum was determined by HPLC,and recoveries of the analyte on MISPE and NISPE were 99.3%and 13.2%,respectively.It was concluded that the dummy template MIP could be used for selective extraction of AZT in serum samples without template bleeding." @default.
- W2371789098 created "2016-06-24" @default.
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- W2371789098 date "2013-01-01" @default.
- W2371789098 modified "2023-09-25" @default.
- W2371789098 title "Dummy-template molecularly imprinted polymer for selective solid-phase extraction of zidovudine in human serum" @default.
- W2371789098 hasPublicationYear "2013" @default.
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