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- W2371805923 abstract "Objective To culture and isolate of Bartonella and analysis of its biological characteristics.Methods Bartonella strains were isolated from 913 rat blood samples collected from 16 counties in Yunnan Province and inoculated into macconkey agar with 5% non-fiber rabbit blood brain heart infusion broth,then cultured and isolated at 35℃ in 5% CO2 chamber,stained with Gram staininig and examined under microscope to observed their characteristics.The suspected bacteria colonies were amplified by PCR with specific Bartonella introducer to amplify specific gene fragment(379 bp of glt A gene),if the objective band appears on electrophoresis chart it can be judged that the bacteria were the positive strains.The ultrastructure of Bartonella from any positive strain was observed with electron microscopy,and the 379 bp fragment of gltA gene was sequenced and compared with other Bartonella strains.Results 251 Bartonella strains were isolated from 913 rat blood samples with the isolation rate of 27.5%.The earliest growing period of positive colonies was 3 days.The morphological changes of positive colony were observed along with the elongation of culturing period with diversity of characteristics.Groups of Bartonella were seen twining round through scanning electron microscopy.Small coccobacillus and thicker membrane of Bartonella were noticed through transmission electron microscopy.The three genotypes were found in the Bartonella in Yunnan by sequencial analysis.Conclusion Bartonella strains are diverse,and its morphology may metamorphose associated with its broad pathogenesis to human.These findings demonstrate that Bartonella spp is highly pprevalent and its genotype is highly diverse.The findings also indicate further investigations be carried out on elucidate its role in the transmission of unknown infection." @default.
- W2371805923 created "2016-06-24" @default.
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- W2371805923 date "2007-01-01" @default.
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- W2371805923 title "Culture and isolation of Bartonella and analysis of its biological characteristics" @default.
- W2371805923 hasPublicationYear "2007" @default.
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