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- W2371809738 abstract "The full length MADS-box gene EnWM8 was isolated from Elymus nutans by homology cloning,and was submitted to GenBank(accession number JF683846).Analyses of the cDNA sequence showed that EnWM8 was 1 196 bp and encoded 275 amino acids,typical of the MIKC-type MADS-box genes with a typical MADS-box domain and K semi-conservative district.It is speculated that it has some connection with flower development,fruit ripening etc.The deduced amino acid sequence showed that there were 95.62%,96.72%,96.28% similarities between the gene and Triticum aestivum genes WM8,AGL29,and fruitful-like.The phylogenetic tree deduced by DNAMAN software indicated that EnWM8 had a close evolutionary relationship with the TaWM8 of T.aestivum,and was distantly related to the AtWM8 and AtAGL29 of Arabidopsis.The WM8 protein of E.nutans is a hydrophilic and basic protein with a molecular weight of 13 726.7 Da: the theoretical isoelectric point is 9.14.It has no membrane structure and is mainly located in the nucleus.There are many α-helix,random coil and extended strand structures.These results are a basis for further research on flower development,seed maturation and other mechanisms of E.nutans at the molecular level." @default.
- W2371809738 created "2016-06-24" @default.
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- W2371809738 date "2012-01-01" @default.
- W2371809738 modified "2023-09-28" @default.
- W2371809738 title "Cloning and analysis of the MADS-box gene WM8 of Elymus nutans" @default.
- W2371809738 hasPublicationYear "2012" @default.
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