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- W2371857701 abstract "High performance liquid chromatography(HPLC) coupled with resonance light scattering(RLS) method were established for determination of the isepamicin sulfate in the injection.The chromatographic separation was performed on a Agilent ZORBAX SB C18(4.6 mm × 250 mm,5 μm) column.The mobile phase was consisted of water∶acetonitrile = 97∶3(φ) at flow rate of 0.5 mL /min,the fluorescence detertor was set at λex= λem= 488 nm,Titan Yellow of 20 μg /mL with the BR solution was chosen as molecular recognition probe,and the flow rate of probe solution was0.20 mL /min at post-column.The concentration of isepamicin in the linear range of 5.0 ~ 30.0 μg /mL had a good linear relationship,the detection limit was 1.0 μg /mL,low,and medium and high three kinds of recoveries are greater than 98%.The method was simple,more accurate and sensitivity,and provided an effective way in quality control of isepamicin sulfate injection." @default.
- W2371857701 created "2016-06-24" @default.
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- W2371857701 date "2014-01-01" @default.
- W2371857701 modified "2023-09-24" @default.
- W2371857701 title "Determination of Isepamicin Sulfate in Injection by HPLC-RLS Method" @default.
- W2371857701 hasPublicationYear "2014" @default.
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