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- W2371877551 abstract "Objective To prepare and identify the particles tumor vaccine based on tumor endothelial marker-8 (TEM-8) and stimulated cytotoxic activity of CTL in vitro. Methods Under specific condition, peptide and DNA were condensed into particle, and the preparing parameters were optimized by assays of DNA gel retardation, DNase I protection, and identified by Western blotting and 51Cr release assay. Results The vaccine was condensed into homogeneous particles at charge ratio of 2 in 87.5 mmol/L NaCl concentration, which effectively medicated the expression of the genes carried by vaccine particles. Conclusion The tumor vaccine based on pTEM-8 is prepared successfully, and induces specific CTL with specific cytotoxic effect. These results provide a good foundation for the design of new tumor vaccine." @default.
- W2371877551 created "2016-06-24" @default.
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- W2371877551 date "2009-01-01" @default.
- W2371877551 modified "2023-09-26" @default.
- W2371877551 title "Preparation and identification of a novel particle vaccine based on pTEM-8 and its ability to stimulate CTL cytotoxic activity" @default.
- W2371877551 hasPublicationYear "2009" @default.
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