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- W2371878234 abstract "Objective To evaluate the immunity of mpt64-recombinant BCG vaccine.Methods The antigen-specific antibody levels of mouse immunized with mpt64-recombinant BCG were detected by ELISA,and multiplication of mouse lymph-cell was analyzed by MTS.Results There was no difference in weight between the mice immunized with saline and BCG,(the control group) and those immunized with mpt-recombinant BCG vaccine,(the experimental group).The serum of mice in each group collected at different time were detected with antigens such as PPD,ESAT6,MPT64,Ag85A,Ag85B protein.The anti-MPT64 specific antibody was only founded in the mice immunized with mpt64- recombinant BCG vaccine.The antibody level of BCG and the experimental group increased on the day and reached the peak on the 45thday after immunization.When the mouse lymph-cells of the experimental group and the controls were stimulated by different antigen,the mean stimulation index was more than 2.0.Conclusion The mpt64-recombinant BCG vaccine and antigen-specific gene of mycobacterium tuberculosis can express specific protein in BCG and stimulate B cell of mouse to produce specific antibody." @default.
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- W2371878234 date "2008-01-01" @default.
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- W2371878234 title "Immunity of mpt64-recombinant BCG vaccine" @default.
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