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- W2371884685 abstract "Objective To obtaining purified p24 gag protein of HIV 1 by a new prokaryotic expressive vector. Methods p24 fragment was amplified by PCR from the HIV 1 HXB2 cDNA template and cloned into the expressive plasmid pTXB1 for constructing the recombinant plasmid pTXB p24. Purified p24 was obtained by affinity column (chitin beads). Molecular weight and purity of p24 were analyzed with SDS PAGE and HPLC, Antigenity was tested with Western blot and colloid gold technique. Results Under the control of phage T7 promoter leading to production of fusion protein p24 Intein CBD in E.coli , stable expression quantity was above 12%. The purity of p24 was more than 96% with HPLC, and it reacted with serum samples from HIV 1 infected subjects when tested by Western blot and colloid gold technique. Conclusion Obtaining purified non fusion p24 gag protein of HIV 1 with a new prokaryotic expressive vector, may reach a purity more than 96%. [" @default.
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- W2371884685 date "2003-06-17" @default.
- W2371884685 modified "2023-09-25" @default.
- W2371884685 title "Obtaining purified p24 gag protein of HIV-1 by using a new prokaryotic expressive vector" @default.
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