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- W2371919209 abstract "Objective To isolate the culture of rat MSC,and constructed the MSC which were transfected by recombinant adenoviral vector carrying IL-12 gene.Methods MSC was isolated and cultured by Percoll's method.Flow cytometry to detect surface markers of CD29,CD44 and CD34.Using RT-PCR and Western Blott methods to detect the expression of IL-12 mRNA and its protein in transfected MSC.Results Growth status of MSC separated by Percoll presented dolicho-fusiform shape uniformly.Surface antigen markers of MSC by using Flow cytometry analysis,the results showed the positive rates of CD29 and CD44 were 97.1% and 98.2%,respectively,while The positive rate of CD34 is only 4.4%.RT-PCR results demonstrated IL-12 gene was expressed in mRNA level,and Western Blott results were in accordance with those of mRNA.Conclusion Using Percoll's method to isolated and cultured MSC is simply.IL-12 gene was expressed by constructed Ad-IL-12-MSC." @default.
- W2371919209 created "2016-06-24" @default.
- W2371919209 creator A5012765423 @default.
- W2371919209 date "2011-01-01" @default.
- W2371919209 modified "2023-09-23" @default.
- W2371919209 title "Expression of IL-12 in MSC infected by recombinant adenoviral vector Ad-IL-12" @default.
- W2371919209 hasPublicationYear "2011" @default.
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