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- W2371940978 abstract "Intracytoplasmic sperm injection(ICSI) is the process in which a high powered microscope is used to inject specially prepared single spermatozoa into a mature oocyte in order to effect fertilization.ICSI is now considered to be a useful procedure,not only for producing live offspring from nonmotile sperm cells but also for generating transgenic animals through sperm-mediated gene transfer.Mouse ICSI was conducted using mouse sperm or sperm which were incubated with GFP gene for 30 minutes.Genomic DNA was extracted from the offspring of the founder mice for PCR and Southern blotting analysis.PCR and Southern blotting analysis showed 3 of 11 mice were transgenic.These results indicated that the high efficiency of transgenic mouse could be produced by ICSI." @default.
- W2371940978 created "2016-06-24" @default.
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- W2371940978 date "2007-01-01" @default.
- W2371940978 modified "2023-09-26" @default.
- W2371940978 title "Production of Transgenic Mice Expressing GFP by Intracytoplasmic Sperm Injection(ICSI)" @default.
- W2371940978 hasPublicationYear "2007" @default.
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