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- W2371941567 abstract "A single strand specific nuclease,nuclease-S1,can cleave supercoiled pBR322DNA at a specific site.The site was found to be in the loop of the cruciform structure insupercoiled DNA.Supercoiled pARIII DNA,a plasmid with a complete repeating unitof the rDNA gene of the silkworm Attacus ricini in pBR322,can also be specificallycleaved by S1 nuclease,since after S1 nuclease cleavage followed by PstI digestionfragments of definite sizes have been seen.A subclone of pARIII having the EcoRI-BamHI fragment of rDNA-pARIV exhibits S1 cleavage sites similar to pARIII.Dueto the relative sensitivity to S1 nuclease,the original S1 cleavage site in pBR322fragment is covered up in pARIII and pARIV.The function of the cruciform struc-ture in the chromatin of eukaryotic cells is being studied further." @default.
- W2371941567 created "2016-06-24" @default.
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- W2371941567 date "1984-01-01" @default.
- W2371941567 modified "2023-09-25" @default.
- W2371941567 title "CRUCIFORM STRUCTURES IN rDNA OF THE SILKWORM ATTACUS RICINI" @default.
- W2371941567 hasPublicationYear "1984" @default.
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