Matches in SemOpenAlex for { <https://semopenalex.org/work/W2371952112> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2371952112 abstract "Objective To establish the MCF-7 cell line which express transmembrane (TM)-TNF-α intracellular domain (TNF-ICD) protein stably and highly, and to investigate the reverse signaling of TM-TNF-α. Methods The vector of pIRES2-EGFP, which contained human TNF-ICD cDNA, was transduced into a human breast tumor cell line MCF-7 with Lipofectamine 2000. The positive cell clone expressing TNF-ICD protein was detected with indirect immunofluorescence method and flow cytometry. The cytotoxicity of TNF-α against the MCF-7 cells expressing TNF-ICD was determined by bioassay. NO production and NF-κB activity were detected by ELISA method. Results The MCF-7 cells transfected with the TNF-ICD gene could express high lever of TNF-ICD protein, which was confirmed by immunofluorescence and FACS. Bioassay shown that the MCF-7 cells expressing TNF-ICD was resisted to the cytotoxicity of TNF-α. The NO production and NF-κB activity of the transfected cells increased significantly. PDTC, a NF-κB inhibitor, could restore efficiently the sensibility of TNF-ICD-transfected MCF-7 cells to TNF-α. Conclusion The establishment of tumor cell line MCF-7 expressing TNF-ICD gene provide a good model for further studying reverse signaling of TM-TNF-α. TM-TNF-α intracellular domain (-76-1) may be an active domain which involved in the reverse signaling of TM-TNF-α, stimulating NF-κB, and resisting to the cytotoxicity of TNF-α." @default.
- W2371952112 created "2016-06-24" @default.
- W2371952112 creator A5067537526 @default.
- W2371952112 date "2007-01-01" @default.
- W2371952112 modified "2023-09-28" @default.
- W2371952112 title "Biologic effects of the transfected Transmembrane TNF-α-intracellular domain on human breast tumor cell line MCF-7" @default.
- W2371952112 hasPublicationYear "2007" @default.
- W2371952112 type Work @default.
- W2371952112 sameAs 2371952112 @default.
- W2371952112 citedByCount "0" @default.
- W2371952112 crossrefType "journal-article" @default.
- W2371952112 hasAuthorship W2371952112A5067537526 @default.
- W2371952112 hasConcept C104317684 @default.
- W2371952112 hasConcept C109316439 @default.
- W2371952112 hasConcept C109541995 @default.
- W2371952112 hasConcept C121608353 @default.
- W2371952112 hasConcept C153911025 @default.
- W2371952112 hasConcept C17991360 @default.
- W2371952112 hasConcept C202751555 @default.
- W2371952112 hasConcept C203014093 @default.
- W2371952112 hasConcept C2778690821 @default.
- W2371952112 hasConcept C2994423619 @default.
- W2371952112 hasConcept C40767141 @default.
- W2371952112 hasConcept C49923114 @default.
- W2371952112 hasConcept C54009773 @default.
- W2371952112 hasConcept C54355233 @default.
- W2371952112 hasConcept C553184892 @default.
- W2371952112 hasConcept C55493867 @default.
- W2371952112 hasConcept C79879829 @default.
- W2371952112 hasConcept C81089528 @default.
- W2371952112 hasConcept C81885089 @default.
- W2371952112 hasConcept C86803240 @default.
- W2371952112 hasConcept C92087593 @default.
- W2371952112 hasConcept C95444343 @default.
- W2371952112 hasConcept C96232424 @default.
- W2371952112 hasConceptScore W2371952112C104317684 @default.
- W2371952112 hasConceptScore W2371952112C109316439 @default.
- W2371952112 hasConceptScore W2371952112C109541995 @default.
- W2371952112 hasConceptScore W2371952112C121608353 @default.
- W2371952112 hasConceptScore W2371952112C153911025 @default.
- W2371952112 hasConceptScore W2371952112C17991360 @default.
- W2371952112 hasConceptScore W2371952112C202751555 @default.
- W2371952112 hasConceptScore W2371952112C203014093 @default.
- W2371952112 hasConceptScore W2371952112C2778690821 @default.
- W2371952112 hasConceptScore W2371952112C2994423619 @default.
- W2371952112 hasConceptScore W2371952112C40767141 @default.
- W2371952112 hasConceptScore W2371952112C49923114 @default.
- W2371952112 hasConceptScore W2371952112C54009773 @default.
- W2371952112 hasConceptScore W2371952112C54355233 @default.
- W2371952112 hasConceptScore W2371952112C553184892 @default.
- W2371952112 hasConceptScore W2371952112C55493867 @default.
- W2371952112 hasConceptScore W2371952112C79879829 @default.
- W2371952112 hasConceptScore W2371952112C81089528 @default.
- W2371952112 hasConceptScore W2371952112C81885089 @default.
- W2371952112 hasConceptScore W2371952112C86803240 @default.
- W2371952112 hasConceptScore W2371952112C92087593 @default.
- W2371952112 hasConceptScore W2371952112C95444343 @default.
- W2371952112 hasConceptScore W2371952112C96232424 @default.
- W2371952112 hasOpenAccess W2371952112 @default.
- W2371952112 hasRelatedWork W1973315280 @default.
- W2371952112 hasRelatedWork W1982121455 @default.
- W2371952112 hasRelatedWork W2075854039 @default.
- W2371952112 hasRelatedWork W2263872871 @default.
- W2371952112 hasRelatedWork W2348428835 @default.
- W2371952112 hasRelatedWork W2357577818 @default.
- W2371952112 hasRelatedWork W2359378103 @default.
- W2371952112 hasRelatedWork W2360212884 @default.
- W2371952112 hasRelatedWork W2361008305 @default.
- W2371952112 hasRelatedWork W2370006513 @default.
- W2371952112 hasRelatedWork W2372918449 @default.
- W2371952112 hasRelatedWork W2374291032 @default.
- W2371952112 hasRelatedWork W2374477424 @default.
- W2371952112 hasRelatedWork W2376061827 @default.
- W2371952112 hasRelatedWork W2377436951 @default.
- W2371952112 hasRelatedWork W2381493416 @default.
- W2371952112 hasRelatedWork W2384132830 @default.
- W2371952112 hasRelatedWork W2393494591 @default.
- W2371952112 hasRelatedWork W269765013 @default.
- W2371952112 hasRelatedWork W2740541442 @default.
- W2371952112 isParatext "false" @default.
- W2371952112 isRetracted "false" @default.
- W2371952112 magId "2371952112" @default.
- W2371952112 workType "article" @default.