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- W2371962547 abstract "Objective To establish a method for isolating and culturing rat bone marrow mesenchymal stem cells(rBMSCs) in vitro and identify the biological properties.Methods BMSCs were isolated from SD rats and expanded by whole bone marrow adherence culture.The growth and morphology of the primary culture and subculture were observed under inverted phasecontrast microscopy and the growth curve was pained by cell counting.The ability of osteoblastic differentiation and adipocytic differentiation was examined.Results After seeded for 24 hours,most cells of primary generation had adhered.The size of BMSCs from the third generation was highly homogeneous,and the adherent cell showed spindle shape,spiral-like arrangement.After about 2 days of adaptation period,the cells were in logarithmic growth phase between 3-7 days,and came into platform phase 7 days later.After osteoblastic induction for 18 days,BMSCs differentiated into bone cells,which showed calcium node in extracellular matrix by alizarin red staining.BMSCs could differentiate into lipocytes. With O red oil dyeing,lipocytes showed many orange fatty follicles after induced for two weeks.Conclusion The culture method by whole bone marrow adherence is simple and quick for obtaining the BMSCs with stable biological properties." @default.
- W2371962547 created "2016-06-24" @default.
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- W2371962547 date "2010-01-01" @default.
- W2371962547 modified "2023-09-28" @default.
- W2371962547 title "Isolation,culture and biological characteristics of bone marrow-derived mesenchymal stem cells" @default.
- W2371962547 hasPublicationYear "2010" @default.
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