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- W2371984889 abstract "AIM To construct the pIg TRⅡ eukaryotic expression vector of the cDNA sequence encoding bioactive extracellular domain of human transforming growth factor beta type Ⅱreceptor (TGF βRⅡ) to form the fusion gene TGF βRⅡ/Fc and to express the chimeric protein TGF βRⅡ/Fc in CHO cells. This work would contribute to the foundation of gene therapy of pulmonary fibrosis. METHODS Total RNA was extracted from human normal lung tissue and subjected to reverse transcription, and then the human TGF βRⅡ cDNA gene was amplified by RT PCR. The PCR product was cloned into pMD18 Tvector and the sequence was confirmed by restriction enzyme digestion and sequencing.Then the specific TGF βRⅡ encoding region fragment was subcloned into pIg plasmid to form the pIg TRⅡ eukaryotic expression vector. The recombinant plasmid was transfected into CHO cells with liposome transfection reagent for transient expression. The expression of the chimeric protein TGF βRⅡ/Fc was identified by immunofluorescent assay and immunoprecipitation technology. RESULTS Restriction enzyme digestion and DNA sequence analysis revealed that the insert fragment was identical to the published TGF βRⅡcDNA sequence. The chimeric protein was detected by cellular immunofluorescence and immunoprecipitation technology. CONCLUSION Construction of the pIg TRⅡ eukaryotic expression vector of the fusion gene TGF βRⅡ/Fc and its expression in CHO cells are both successful." @default.
- W2371984889 created "2016-06-24" @default.
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- W2371984889 date "2002-01-01" @default.
- W2371984889 modified "2023-09-28" @default.
- W2371984889 title "Construction of eukaryotic expression vector of fusion gene TGF-βRII/Fc and its expression in CHO cells" @default.
- W2371984889 hasPublicationYear "2002" @default.
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