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- W2372005692 abstract "Several genotypes of winter wheat (Triticum aestivum) were chosen for microspore embryogenesis and plant regeneration by cold pretreatment (4 ℃, 3 weeks, for spike in vitro), heat shock and starvation (33 ℃, 4 d , in a starvation medium B for excised anthers from cold-treated spikes). The results were showed below: In the six tested genotypes, embryos were all obtained from microspores, and 107.2 embryos were got per spike in the best genotype of F1-2; The microspore embryogenesis could be induced in cold pretreatment in only five tested genotypes; Much more microspore embryos were produced via heat shock and starvation than that of cold pretreatment, but these embryos were hard to regenerate later in A2R regeneration medium and most of the regeneration plants were albinos; And contrarily embryos triggered by cold pretreatment developed normally and rapidly, and most formed green plants in A2R medium. Thus, when isolated microspore culture was applied for doubled haploid breeding in wheat, cold pretreatment was preferred." @default.
- W2372005692 created "2016-06-24" @default.
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- W2372005692 date "2004-01-01" @default.
- W2372005692 modified "2023-09-25" @default.
- W2372005692 title "Effect of Cold, Heat Shock and Starvation on Microspore Embryogenesis and Regeneration in Winter Wheat" @default.
- W2372005692 hasPublicationYear "2004" @default.
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