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- W2372019566 abstract "A 1.5Kb of 16SrDNA fragment amplified through gen eral PCR with the template of field bacterium F0303 total DNA,and bacteria 16SrDNA universal primers,was ligated into pGEM-Teasy vector which is classic cloning vector for PCR products,and then was taken to transform chemically competent cell of E.coli DH5α.Restriction enzyme analysis of the 5 randomly selected transformants,mediated by EcoR I,demonstrates they were all positive.The BLAST of the 1475 bp of 16SrDNA sequence obtained by DNA sequencing,with all 16SrDNA sequences in GenBank indicated that the field bacterium F0303 belongs to Serratia genus and the phylogenic tree of the field bacterium F0303,which was generated by Vector NTI Suite 6 software,basing on 16SrDNA full-length sequence,showed that it is most close to Serratia rubidaea with 97.8% of identity." @default.
- W2372019566 created "2016-06-24" @default.
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- W2372019566 date "2005-01-01" @default.
- W2372019566 modified "2023-09-25" @default.
- W2372019566 title "16SrDNA sequence analysis and phylogenic tree construction of a strain of field bacterium" @default.
- W2372019566 hasPublicationYear "2005" @default.
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